Journal: Clinical Cancer Research

Article Title: A Phase II Study of Lapatinib in Recurrent/Metastatic Squamous Cell Carcinoma of the Head and Neck

doi: 10.1158/1078-0432.ccr-11-2825

Figure Lengend Snippet: Figure 1. Kaplan–Meier survival curves. Survival estimates of PFS and OS for patients who were EGFR naive (group A) and those with prior exposure to EGFR (group B).

Article Snippet: To measure potential target inhibition by lapatinib and downstream signaling, antibodies against human phosphorylated EGFR (pEGFR; Y1173; Santa Cruz Biotechnology; 1:100), EGFR (1005; Santa Cruz; 1:100), pERK (Calbiochem; 1:100), ERK (Calbiochem, 1:100), pAKT (Calbiochem catalog# 1240011:100), and AKT (Cell Signaling catalog# 2966;1:100) were incubated with the rehydrated slides as previously described (21).Quantification of protein expression was determined by laser scanning cytometry (LSC) analysis which obtains 2- and 3-color mean fluorescence intensity (MFI) information as previously described (21, 22).

Techniques:

Journal: Clinical Cancer Research

Article Title: A Phase II Study of Lapatinib in Recurrent/Metastatic Squamous Cell Carcinoma of the Head and Neck

doi: 10.1158/1078-0432.ccr-11-2825

Figure Lengend Snippet: Figure 2. Ratio of MFI of selected biomarkers by LSC pre- and posttreatment with lapatinib. Posttreatment biopsies showed a significant decreased pERBB2/ ERBB2 (or pHER-2/HER-2) ratio when compared with pretreatment biopsies. Pre- and posttreatment pEGFR/EGFR ratios were not statistically different. Five patients were analyzed pre- and posttreatment. Nine patients were analyzed pretreatment. Stable disease was the best response in one patient.

Article Snippet: To measure potential target inhibition by lapatinib and downstream signaling, antibodies against human phosphorylated EGFR (pEGFR; Y1173; Santa Cruz Biotechnology; 1:100), EGFR (1005; Santa Cruz; 1:100), pERK (Calbiochem; 1:100), ERK (Calbiochem, 1:100), pAKT (Calbiochem catalog# 1240011:100), and AKT (Cell Signaling catalog# 2966;1:100) were incubated with the rehydrated slides as previously described (21).Quantification of protein expression was determined by laser scanning cytometry (LSC) analysis which obtains 2- and 3-color mean fluorescence intensity (MFI) information as previously described (21, 22).

Techniques: